简要描述：BD Purified Rat Anti-Mouse CD16/CD32 553141，BD 553141 Pharmingen 纯化大鼠抗小鼠 CD16/CD32 抗体，常用于流式细胞术和免疫组织化学等实验中，作为 BD Fc 阻滞剂。该抗体通过阻断小鼠 Fc 受体（CD16/CD32）与 Fc 区域结合，防止非特异性结合，从而提高下游实验中抗体标记的特异性和准确性。

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更新时间：2025-06-10
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产品介绍

品牌	BD/美国	货号	553141
规格	0.1mg	供货周期	两周

BD Purified Rat Anti-Mouse CD16/CD32 553141

纯化大鼠抗小鼠CD16/CD32小鼠Fc阻滞剂

Brand:BD Pharmingen™

Alternative Name:FcγRIII/FcγRII; Fcgr3/Fcgr2

Reactivity:Mouse (QC Testing)

Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ

Immunogen:Mouse BALB/c Macrophage J774

Application:Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)

Concentration:0.5 mg/ml

Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status:RUO

BD Purified Rat Anti-Mouse CD16/CD32 553141

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

推荐的实验流程

To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent.

To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis:

1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4˚C for 5 minutes.

2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells).

3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG2b, κ).

BD Purified Rat Anti-Mouse CD16/CD32 553141